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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in advertising and marketing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been reported but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specifically to your target antibody and block antibody binding. These peptide generally has the epitope identified through the antibody.
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This guidebook outlines more feasible optimization of cellular and immunoassay parameters to be certain the best possible effects are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining through the blocked antibody as opposed to the antibody alone, one can see which staining is distinct.
Concentrate on Details Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation patterns which lead to your temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and capabilities being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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